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Růst Mycobacterium smegmatis na agarovém médiu a agarovém médiu pokrytém celofánovou folií - morfologická a proteomová studie
Ramaniuk, Volha ; Weiser, Jaroslav (advisor) ; Beranová, Jana (referee)
Biofilm formation is one of the most common bacterial survival strategies. Majority of bacterial species are able to form these three-dimensional structures, including pathogens like Mycobacterium tuberculosis. Representatives of Mycobacterium genus widely occur in the nature, although they can cause serious problems when they appear in medical equipment and artificial replacements of the human body. Non-pathogenic Mycobacterium smegmatis mc2 155 was used as a model organism in our experiments. We investigated morphology of the three- and six-day-old colonies (in fact biofilms) on agar and agar covered with cellophane using Stereo microscope and Scanning Electron Microscope. We found that a type of surface as well as a carbon source has a great influence on the morphology of the M. smegmatis colonies. We isolated proteomes from the agar and cellophane cultures and from planktonic culture. Two-dimensional electrophoresis was used as the main proteomic method. Proteomic data were analyzed using PDQuest software. Then the sets of proteins detected by qualitative and quantitative analyses were compared using Venn diagrams. As a result, we recognized 7 unique proteins that might be specific for recognition and adhesion of bacteria to the cellophane, no unique protein in agar proteome and 46 unique...
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Venn diagrams - Teaching Mathematics using the CLIL method for upper secondary pupils
Vojířová, Barbora ; Novotná, Jarmila (advisor) ; Moraová, Hana (referee)
This thesis focuses on the teaching of mathematics using the CLIL method. The topic is Venn diagrams and learning how to solve problems using these diagrams. The language chosen for the CLIL method is English. First, the CLIL method is described, and later I introduce sets and Venn diagrams. The aim of the thesis is to prepare and implement two lessons using the CLIL method in English, and two lessons in Czech, followed by preparing a test in both languages for a third lesson. The thesis was realized by conducting a teaching experiment, which took place in two classes of multi- year grammar school (in the Quintas - the first year of secondary school). I have compiled and tested four worksheets - two with Czech tasks, two with English tasks, a presentation for the lesson using the CLIL method, and tests for both language versions. Both the tasks and solutions are part of the thesis. Before the preparation of the thesis, the following two research questions were identified: 1. What needs to be taken into consideration when preparing for the Venn diagrams lesson using the CLIL method, and what needs to be added to this lesson compared to the Czech lesson? 2. At what success rate will pupils learn using the CLIL method in English and pupils using Czech, with regard to equation building and answers to...
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Růst Mycobacterium smegmatis na agarovém médiu a agarovém médiu pokrytém celofánovou folií - morfologická a proteomová studie
Ramaniuk, Volha ; Weiser, Jaroslav (advisor) ; Beranová, Jana (referee)
Biofilm formation is one of the most common bacterial survival strategies. Majority of bacterial species are able to form these three-dimensional structures, including pathogens like Mycobacterium tuberculosis. Representatives of Mycobacterium genus widely occur in the nature, although they can cause serious problems when they appear in medical equipment and artificial replacements of the human body. Non-pathogenic Mycobacterium smegmatis mc2 155 was used as a model organism in our experiments. We investigated morphology of the three- and six-day-old colonies (in fact biofilms) on agar and agar covered with cellophane using Stereo microscope and Scanning Electron Microscope. We found that a type of surface as well as a carbon source has a great influence on the morphology of the M. smegmatis colonies. We isolated proteomes from the agar and cellophane cultures and from planktonic culture. Two-dimensional electrophoresis was used as the main proteomic method. Proteomic data were analyzed using PDQuest software. Then the sets of proteins detected by qualitative and quantitative analyses were compared using Venn diagrams. As a result, we recognized 7 unique proteins that might be specific for recognition and adhesion of bacteria to the cellophane, no unique protein in agar proteome and 46 unique...
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Venn diagrams
TOMANOVÁ, Denisa
The aim of the bachelor thesis is to create a list of mathematical problems dealing with the representation of sets in Venns diagrams. The overview serves as an inspiration for teachers and includes the main types of examples to practice. The examples are designed and intended for use in primary schools. The first part of the thesis is theoretical. This section is focused on the description set and subsequent display sets to Venns diagrams. The second part of the thesis deals with Venns diagrams in practice. There are solid examples given in five sub-sections. These examples show the use of Venns diagrams in roles of various types.
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